学院：理工学院
性别：男
职称：教授
职务：食品安全与营养研究院院长
学历：研究生
学位：博士
学习经历:
1981 .1-1985.6 河北大学生物系微生物及生化专业理学学士
1996 .1－1997 .3 日本国立冈山大学药学院研究生（日本政府奖学金留学生）
1997 .4－2000 .3 日本国立冈山大学药学院博士（日本政府奖学金留学生）
工作经历:
1985.08-1995.12：河北省疾病预防控制中心传染病防治科主管
2000.04 - 2000.09：日本冈山大学药学部研究员
2000.10 - 2001.03：日本国立国际医疗中心研究员
2000.10-2003.03：日本人类科学财团研究员
2001.04-2003：03日本大阪府立大学研究员
2005.04-起：日本大阪府立大学研究员
2010.06起：肉食品安全生产技术国家重点实验室主任
2016.01起：暨南大学食品与营养研究院院长，博士生导师
研究方向:
食品安全快速检测与食源性微生物
承担及参与项目
1.国家973计划项目《肉品质量与安全控制技术的关键科学问题研究》（2010CB35704）。(2011年1月-2012年12月)。250万元
2.国家973计划项目《肉食品中微生物控制关键技术研究》（2012CB723705）。(2012年1月-2013年12月)。300万元
3.厦门市重大科技计划项目《肉品安全快速基因检测方法的研究》（3502Z 20110001）。(2011年1月-2012年12月)。200万元
4.国家科技支撑计划课题。项目名称:畜禽产品安全生产技术集成与示范。课题名称:猪肉安全生产技术集成与示范。（2014BAD13B00-01）。(2014年1月-2016年12月)。400万元
5.国家海洋经济创新发展区域示范专项。高灵敏度和高特异性的海洋病毒与细菌快速诊断试剂盒成果转化示范.。（ GD2012-B03-003）(2013年1月-2015年12月)。820万元
6.国家“十二五”科技重大专项。专项名称：艾滋病和病毒性肝炎等重大传染病防治。课题名称：结核病新型诊断产品研发及其产业化。（2013ZX10003001）(2013年1月-2015年12月)。1700万元
7.高等学校科技创新工程重大项目培育资金项目。课题名称: 基于siRNA干扰的整合子耐药基因沉默研究。(706046) (2007年1月-2009年12月)。30万元
8.国家自然科学基金面上项目。课题名称:抗生素诱导整合子系统捕获耐药基因机制研究（20877028）(2009年1月-2011年12月)。29万元
代表性论文
2015年
1.Liu, ZB ; Zhang, ZG ; Yan, H ; Li, JR ; Shi, L*.Isolation and molecular characterization of multidrug-resistant enterobacteriaceae strains from pork and environmental samples in Xiamen, China. Food Protection.2015, 78(1):78-88.
2.Di, HL ; Ye, L ; Neogi, SB ; Meng, HC ; Yan, H ;Yamasaki, S ; Shi, L*.Development and evaluation of a loop-mediated isothermal amplification assay combined with enrichment culture for rapid detection of very low numbers of vibrio parahaemolyticus in seafood samples. Biological &pharmaceutical Bulletin. 2015, 38(1):82-87.
2014年
1.Di, HL ; Ye, L ; Yan, H; Meng, HC ; Yamasak, S ; Shi, L*.Comparative analysis of CRISPR loci in different Listeria monocytogenes lineages. Biochenmical and Biophysical Research Communications. 2014,454(3):399-403.
2.Xu, DY ; Li, YL ; Zahid, MSH ; Yamasaki, S ; Shi L; Li, JR ; Yan, H .Benzalkonium chloride and heavy-metal tolerance in Listeria monocytogenes from retail foods.International Journal of Food Microbiology. 2014,190:24-30.
3.Jiang, XB; Yu, T ; Wu, N ; Meng, HC ; Shi, L*.Detection of qnr, aac(6 ')-Ib-cr and qepA genes in Escherichia coli isolated from cooked meat products in Henan, China. International Journal of Food Microbiology. 2014,187:22-25.
4.Li, YM ; Shi L; Pan, AQ ; Cao, WW ; Chen, X ; Meng,HC; Yan, H ; Miyoshi, S ; Ye, L.Evaluation of real-time loop-mediated isothermal amplification (RealAmp) for rapid detection of mycobacterium tuberculosis from sputum samples. Journal of Microbiological Methods. 2014,104:55-58.
5.Yi, MY; Ling, L ; Neogi, SB ; Fan, YS ; Tang, DY ; Yamasaki, SJ ; Shi, L ; Ye, L . Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus. Food Control. 2014,41:91-95.
6.Ding, X; Nie, K ; Shi, L ; Zhang, Y ; Guan, L ; Zhang, D ; Qi, SX ; Ma, XJ.Improved detection limit in rapid detection of human Enterovirus 71 and Coxsackievirus A16 by a novel reverse transcription-isothermal multiple-self-matching-initiated amplification assay. Journal of Clinical Microbiology. 2014,52(6):1862-1870.
7.Zeng, YL; Zhang, XG ; Nie, K ; Ding, X ; Ring, BZ ; Xu, LY ; Dai, L ; Li, XY; Ren, W ; Shi, L*. Rapid quantitative detection of Human immunodeficiency virus type 1 by a reverse transcription-loop-mediated isothermal amplification assay. Gene. 2014,541(2):123-128.
8.Yang, Y ; Zhou, CQ ; Shi, L ; Meng, HC; Yan, H.Prevalence and characterization of drug-resistant tuberculosis in a local hospital of Northeast China. International journal of infectious diseases. 2014, 22:83-86.
9. Lu,X ; Li,XB ; Mo, ZY; Jin, FG ; Wang,BL ; Huang, JF; Huang, JC ; Zhao, HB ; Shi, L*.
Chikungunya emergency in China: microevolution and genetic analysis for a local outbreak. Virus Genes. 2014,48(1):15-22.
2013年
1.Ye, L ; Zhang, L ; Li, XH ; Shi, L; Huang, Y ; Wang, HH .Antibiotic-Resistant Bacteria Associated with Retail Aquaculture Products from Guangzhou, China. Food Protection.2013,76(2):295-301
2.Bi, S. L.; Shi, L.; Yan, H.; Meng, H. C., Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples. Ann. Microbiol. 2013, 63 (1): 179-185;
3. Feng, J. L.; Lin, J. Y.; Jiang, X. B.; Zhang, O.; Zhu, J. F.; Hu, J.; Shi, L.; Chen, Q., Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers' diarrhoea. Journal Of Medical Microbiology .2013, 62: 701-707;
4.Jiang, X. B.; Shi, L*., Distribution of tetracycline and trimethoprim/sulfamethoxazole resistance genes in aerobic bacteria isolated from cooked meat products in Guangzhou, China. Food Control .2013, 30 (1): 30-34;
5.Wang, L.; Shi, L.; Su, J. Y.; Ye, Y. X.; Zhong, Q. P., Detection of Vibrio parahaemolyticus in food samples using in situ loop-mediated isothermal amplification method. Gene. 2013, 515 (2):421-425.
2012年
1.Zhu X, Wang S, Gu Y, Li X, YanH, Miyoshi S , and Shi L*.Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE. World J Microbiol Biotechnol .2012, 28:2229–2236
2.Shan X, Zhang Y, Zhang Z, Chen M, Su Y, Yuan Y, Alam MJ, Yan H, and Shi L*. Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification. Food Sci. Biotechnol. 2012, 21(1): 101-106
3.Zhang Y, Shan X, Shi L, Lu X, Tang X, Wang Y, Li Y, Alam MJ, and Yan H*.Development of a fimY-based loop-mediated isothermal amplification assay for detection of Salmonella in food. Food Research International. 2012,45: 1011–1015
2011年
1.Ye Y, Wang B, Huang F, Song Y, Yan H, Alam MJ, Yamasaki S, and Shi L*. Application of in situ loop-mediated isothermal amplification method for detection of Salmonella in foods. Food Control. 2011, 22:438-444.
2.Feng J, Yan Y, Chowdhury N, Neogi S, Yamasaki S, Shi L, Hu J, Chen Q*. Identification and characterization of Integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China. International Journal of Food Microbiology. 2011，144:337–341
3.Xu Z, Li L, Shi L, and Shirtliff ME*. Class 1 integron in staphylococci. Molecular Biology Reports. 2011 , 38(8):5261-5279
4.Bi S, Yan H, Chen M, Zhang Z, Shi L*, and Wang H. New variant Salmonella genomic island 1-U in Proteus mirabilis clinical and food isolates from South China. J Antimicrob Chemother. 2011, 66(5):1178-1179.
5.Xu Z, Li L, Shirtliff M, Peters B, Li B, Peng Y, Alam MJ, Yamasaki S, and Shi L*. Resistance class 1 integron in clinical methicillin-resistant Staphylococcus aureus strains in southern China, 2001–2006. Clinical Microbiology and Infection. 2011, 17(5):712-724.
6.Lu X, Mo ZY*, Zhao HB, Yan H, and Shi L. LAMP-based method for a rapid identification of Legionella spp. and Legionella pneumophila. Appl Microbiol Biotechnol .2011, 92:179–187
7.Meng HC, Zhang ZG, Chen MR, Su YY, Li L, Miyoshi S, Yan H*，and Shi L*.Characterization and horizontal transfer of class 1 integrons in Salmonella strains isolated from food products of animal origin. International Journal of Food Microbiology. 2011, 149: 274–277
2010年
1.Yan H, Li L, Alam MJ, Shinoda S, Miyoshi S*, and Shi L*. Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China. International Journal of Food Microbiology. 2010, 143(3): 230–234
2.Yan H, Neogi S, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L*, and Zhong N. Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005–2007. International Journal of Food Microbiology. 2010, 144:310–316
3.Xu Z, Li L, Shirtliff M, Peters B, Peng Y, Alam MJ, Yamasaki S, and Shi L*. First report of class 2 integron in clinical Enterococcus faecalis and class 1 integron in Enterococcus faecium in South China. Diagnostic Microbiology and Infectious Disease. 2010, 68:315–317
4.Zhang X*, Wang W, Xiao K, and Shi L. Translational medicine: Application of omics for drug target discovery and validation. Springer Science. 2010, Chapter 13, 235-247.
5.Yan, H., Li, L., Zong, M., Alam, M.J., Shinoda, S., Shi, L*. Occurrence and characteristics of Class 1 and 2 Integrons in clinical bacterial isolates from patients in South China. Journal of Health Science 2010,56, 442-450.
2009年
1.Lu Y, Yang W, Shi L , Li L, Alam MJ, Guo S, and Miyoshi S*. Specific detection of viable Salmonella cell by an Ethidium monoazide-Loop mediated isothermal amplification (EMA-LAMP) method. Journal of Health Science. 2009, 55(5):820-824.
2.Liu F, Guan W, Alam MJ, Shen Z, Li L, Shinoda S, and Shi L*. Pulsed-field gel electrophoresis typing of multidrug-resistand Vibrio parahaemolyticus isolated from various sources of seafood. Journal of Health Science. 2009, 55(5):783-789.
3.Xu Z, Li L, Shirtliff ME, Alam MJ, Yamasaki S, and Shi L* . Occurrence and Characteristics of Class 1 and 2 Integrons in Pseudomonas aeruginosa Isolates from Patients in Southern China. Journal of Clinical Microbiology, 2009, 47(1):230–234.
2008年
1.Xu Z, Li L, Alam MJ, Zhang L, Yamasaki S, Shi L*. First confirmation of integron-bearing methicillin-resistant Staphylococcus aureus. Curr Microbiol. 2008;57(3):264-8.
2.Wang L, Li L, Alam MJ, Geng YH, Li ZY, Yamasaki S and Shi L*. Loop-mediated isothermal amplification method for rapid detection of the toxic dinofagellate Alexandrium ,which causes algal bloomsand poisoning of shellish. FEMS Microbiol Lett. 2008, 278: 1-7.
3.Wang L, Shi L*, Alam MJ, Geng YH, Li L. Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification method. Food Research International. 2008. 41(1):69-74.
4.Yan H, Shi L, Alam MJ, Li L, Yang LS and Yamasaki S *. Usefulness of Sau-PCR for molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia which occurred in a local hospital in Guangzhou, China. Microbiology and Immunology. 2008, 52(5):283-286.
5.Xu Z, Shi L*, Alam MJ, Li L and Yamasaki S. Integron－bearing methicillin － resistance staphylococci in South China, 2001 － 2004. FEMS Microbiol Lett. 2008, 278(2): 223-230.
2007年
1.Xu Z, Shi L*, Zhang C, Zhang L, Li X, Cao Y, Li L and Yamasaki S. Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China. Clinical Microbiology and Infection, 2007, 13 (10). 980-984.
2.Asakura M, Hinenoya A, Alam M, Shima K, Zahid HS, Shi L, Sugimoto N, Ghosh A. N, Ramamurthy T, Faruque MS, Nair G, and Yamasaki S*. An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli. PNAS. 2007, 104 (36): 14483–14488.
3.Shi L, Kou Y, Li L, Miyoshi S*.Analysis of genetic determinants involved in multi-resistance in clinical strains isolated from renal transplantation recipients in Guangzhou, China. Journal of Health Science. 2007, 53(2):185-189.
4.Yan H, Shi L, Yamasaki S, Li XH, Cao YC, Li L, Yang LS and Miyoshi S*. A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 5 and nonsense-mutated cmlA1 gene cassettes. Journal of Health Science. 2007,53（6）:750-755.
2006年
1.Li X, Shi L*, Yang W, Li L, Yamasaki S. A new array of aacA4-catB3-dfrA1 gene cassettes and a none-ORF cassette from a class 1 integron-positive clinical strain of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy. 2006,55(6): 2278-2279.
2.Su J, Shi L*,Yang L, Xiao Z, Li X, Yamasaki S. Analysis of integrons in clinical isolates of Escherichia coli in China during the last six years. FEMS Microbiology Letters.2006, 254(1): 75-80.
3.Shi L, Sato T, Fujihara K, Ito H, Garg P, Chakrabarty R, Ramamurthy T, Nair GB, Takeda Y,Yamasaki S*. Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrheal patients between 1992-2000 in India. Journal of Medical Microbiology. 2006, 55:575-583.
4.Shi L, Zheng MP, Xiao ZH, Asakura M, Su JY, Li L,Yamasaki S*. Unnoticed Spread of Class 1 Integrons in Gram-positive Clinical Strains Isolated in Guangzhou, China. Microbiology and Immunology . 2006,50(6): 463－467.
*: Corresponding author
部分授权专利：
1.一种耐利福平结核分枝杆菌的诊断方法（专利号：2011102463610）
2.一种快速鉴定结核分枝杆菌与非结核分枝杆菌的方法（专利号：201110246388X）
3.一种基孔肯雅病毒等温扩增检测试剂盒及其引物（专利号：2011103019932）
4.转基因玉米MIR604及其衍生品种的LAMP检测引物组、检测试剂盒及检测方法（专利号：2012101287620）
5.转基因大豆A5547-127及其衍生品种的LAMP检测引物组、检测试剂盒及检测方法（专利号：2012101287921）
6.转基因大豆GTS40-3-2的LAMP检测引物组、试剂盒及检测方法（专利号：2012101288017）
7.转基因大豆DP-305423及其衍生品种的LAMP检测引物组、检测试剂盒及检测方法（专利号：201210128764X）
8.转基因水稻BT63及其衍生品种的LAMP检测引物组、检测试剂盒及检测方法（专利号：2012101287940）
9.转基因玉米Event98140及其衍生品种的LAMP检测引物组、检测试剂盒及检测方法（专利号：2012101288036）
10.转基因玉米BT176及其衍生品种的LAMP检测引物组、试剂盒及检测方法（专利号：2012101288021）
11.鲤春病毒血症病毒（SVCV）的RT-LAMP检测引物组、试剂盒及检测方法（专利号：2012105146994）
部分受理专利：
1.军团菌恒温基因扩增快速检测试剂盒及检测方法（专利受理号：2008102193520）
2.结核分枝杆菌恒温基因扩增快速检测试剂盒及检测方法（专利受理号：2008102193516）
3.恒温基因扩增检测转基因玉米Mon89034及其衍生品种的试剂盒及方法（专利受理号：2011101186822）
4.一种恒温实时荧光扩增技术（专利受理号：2012102388864）
5.对虾白斑综合症病毒（WSSV）的LAMP检测引物组及试剂盒（专利受理号：2013104854001）
6.食用油中转基因成分CaMV35S的恒温检测方法和检测试剂盒（专利受理号：2013106108886）
7.检测禽流感病毒H7N9亚型的RT-LAMP试剂盒（专利受理号：201410015168X）
8.一种基于恒温技术检测结核分枝杆菌复合群的方法和检测试剂盒（专利受理号：2014101054193）
9.可常温运输的干粉化恒温扩增检测试剂及其制备方法（专利受理号：2014101730603）
10.外观专利：恒温荧光检测仪（专利受理号：201430145614X）
11.一种快速鉴定结核分枝杆菌死活的方法（专利受理号：2011102463095）